Figure 6

stag1a and stag2b mutations differentially alter the production of primitive myeloid cells in the anterior lateral mesoderm at 12 hpf. (A)scl expression in whole-mount embryos at 12 hpf. Ventral views of ALM are shown; dorsal to the top. scl expression is comparable to wildtype in stag1a^nz204 homozygous mutant embryos and reduced in stag2b^nz207 homozygous mutant embryos. (B)scl expression in whole-mount embryos at 15 hpf. Top panels show lateral views and bottom panels show ventral views of the ALM. Expanded scl expression in the ALM of stag1a^nz204 mutants is rescued upon injection of functional stag1a mRNA. The reduced scl expression in the ALM of stag2b^nz207 mutants is rescued upon injection of functional stag2b mRNA. Changes in expression are marked by arrows and the number of embryos is indicated below each panel. (C) Multiplexed in situ HCR of runx1 and spi1 at 15 hpf. Maximum intensity projections of a single ALM stripe; lateral views with dorsal to the top. Expression domains of runx1 (Alexa Fluor 647) and spi1 (Alexa Fluor 514, false color blue) broadly overlap in all embryos. Both runx1 and spi1 are expanded in stag1a^nz204 embryos but reduced in stag2b^nz207 embryos. Changes in expression are indicated by white arrows. Scale bars are 10 μm. The number of embryos analyzed is indicated below the respective panels.