FIGURE

Figure 4

ID

ZDB-FIG-201213-4

Publication

Oel et al., 2020 - Nrl Is Dispensable for Specification of Rod Photoreceptors in Adult Zebrafish Despite Its Deeply Conserved Requirement Earlier in Ontogeny

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Figure 4

Nrl is Dispensable for Rod Specification in Adult Zebrafish

(A) Monitoring for appearance of GFP-positive rods during ontogeny of nrl^−/− larvae directed our attention to 11 days postfertilization (dpf), where rods are sporadically detectable, but varied between individuals and between eyes of the same individual. Wild-type (WT) eyes at top with abundant rods provided for context.

(B–D) Adult nrl^−/− zebrafish possess a large abundance of rods indistinguishable from WT, such that GFP-positive rods are obvious in intact animals (B) or retinal cryosections (C). Adult nrl^−/− retina showed normal distribution of rod outer segments (ros) apical of rod cell bodies in the outer nuclear layer (ONL, also in [D]). Inner nuclear layers (INLs) and retinal ganglion cell layers (RGCs) are overtly normal (quantified in Figures S5G and S5H).

(E) Immunolabelling with rod-specific 4C12 and anti-UV-opsin confirms presence of rods and normal UV cones, respectively, in adult nrl^−/− retina.

Expression Data

Gene:	EGFP
Antibodies:	Ab-4C12 Ab1-10C9.1
Fish:	nrl^{ua5009/ua5009}; kj2Tg/kj2Tg
Anatomical Terms:	retinal rod cell short single cone cell
Stage Range:	Days 7-13 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nrl^{ua5009/ua5009}; kj2Tg/kj2Tg
Observed In:	retina retinal rod cell short single cone cell
Stage Range:	Days 7-13 to Adult

Phenotype Detail

Acknowledgments

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