Figure 4

Figure 4

Nrl is Dispensable for Rod Specification in Adult Zebrafish

(A) Monitoring for appearance of GFP-positive rods during ontogeny of nrl^−/− larvae directed our attention to 11 days postfertilization (dpf), where rods are sporadically detectable, but varied between individuals and between eyes of the same individual. Wild-type (WT) eyes at top with abundant rods provided for context.

(B–D) Adult nrl^−/− zebrafish possess a large abundance of rods indistinguishable from WT, such that GFP-positive rods are obvious in intact animals (B) or retinal cryosections (C). Adult nrl^−/− retina showed normal distribution of rod outer segments (ros) apical of rod cell bodies in the outer nuclear layer (ONL, also in [D]). Inner nuclear layers (INLs) and retinal ganglion cell layers (RGCs) are overtly normal (quantified in Figures S5G and S5H).

(E) Immunolabelling with rod-specific 4C12 and anti-UV-opsin confirms presence of rods and normal UV cones, respectively, in adult nrl^−/− retina.