FIGURE

Figure EV5

ID

ZDB-FIG-201116-17

Publication

Whittle et al., 2020 - MYH3-associated distal arthrogryposis zebrafish model is normalized with para-aminoblebbistatin

Other Figures

All Figure Page

Back to All Figure Page

Figure EV5

Tricaine fails to ameliorate the <italic>smyhc1</italic><sup>R673H</sup> larval phenotype, and blebbistatin improves the <italic>smyhc1</italic><sup>R673H</sup> larval phenotype

Zebrafish embryos (2 dpf) treated with 40 mg/ml tricaine methanylsulfonate or control egg medium for 24 h starting at 1 dpf. Tricaine suppressed movement but failed to normalize notochord kinks and bends. Tricaine appears to have no major effect on anatomy.

Zebrafish embryos (2 dpf) were treated with 25 μM blebbistatin or DMSO control for 24 h starting at 1 dpf. Blebbistatin appears to partially rescue smyhc1^R673H phenotype, but causes known off target effects. Note the severe dorsal tail curve and pericardial edema present in all larvae treated with blebbistatin regardless of genotype. Representative images of embryos at 48 hpf.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.