FIGURE

Fig. 7

ID

ZDB-FIG-201112-10

Publication

Zhang et al., 2020 - Anti-Müllerian hormone (Amh/amh) plays dual roles in maintaining gonadal homeostasis and gametogenesis in zebrafish

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Fig. 7

Expression of pituitary gonadotropins (fshb and lhb) and gonadal development in juveniles. (A) Expression of fshb and lhb in the control (amh+/−) and mutant (amh−/−) fish at 45 dpf. (B) Quantification of testis size at 50 dpf. Each fish was sectioned serially and longitudinally and the section that showed the largest testis area was chosen for quantification by the ImageJ software (mm2). Different letters indicate statistical significance (P < 0.05; n = 5–8). The representative section of each genotype is shown in C. (C) Testes of different genotypes at 50 dpf. Compared to the control (amh+/−;fshb+/−), the fshb deficiency (amh+/−;fshb−/−) led to hypotrophy of testis and delayed spermatogenesis, whereas amh deficiency (amh−/−;fshb+/−) led to hypergrowth of testis and dysfunctional spermatogenesis. However, double mutation of fshb and amh (amh−/−;fshb−/−) showed antagonistic effects on testis growth with dysfunctional spermatogenesis. (D) Ovaries of different genotypes at 50 dpf. Mutation of fshb delayed PG-PV transition or puberty onset, whereas amh mutation had no effect. The standard body length (cm) and body weight (mg) are indicated for each fish. PG, primary growth follicles; Asterisk, PV follicles; ** P < 0.01.

Expression Data

Genes:	fshb lhb
Fish:	amh^umo17/umo17 amh^umo17/+
Anatomical Terms:	hypophysis female organism hypophysis male organism
Stage:	Days 45-89

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	amh^umo17/umo17 fshb^umo1/+; amh^umo17/+ fshb^umo1/+; amh^umo17/umo17 fshb^umo1/umo1; amh^umo17/+ fshb^umo1/umo1; amh^umo17/umo17
Observed In:	female organism developmental process involved in reproduction female organism follicle cell of egg chamber development hypophysis male organism spermatogenesis testis
Stage:	Days 45-89

Phenotype Detail

Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 517, Zhang, Z., Zhu, B., Chen, W., Ge, W., Anti-Müllerian hormone (Amh/amh) plays dual roles in maintaining gonadal homeostasis and gametogenesis in zebrafish, 110963, Copyright (2020) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.