FIGURE

FIGURE 6

ID

ZDB-FIG-201007-19

Publication

Wang et al., 2020 - A Mutation in VWA1, Encoding von Willebrand Factor A Domain-Containing Protein 1, Is Associated With Hemifacial Microsomia

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FIGURE 6

Proliferation and apoptosis of cranial neural crest cells at approximately 30 hpf. (A) Immunofluorescence results showing anti-phosphohistone H3 (PHH3) staining demonstrating cell proliferation. Merged image demonstrates higher anti-PHH3 signals in cranial neural crest cells compared with uninjected embryos. Arrows: pharyngeal arches. (B) Apoptosis assessed by immunofluorescence detection of TUNEL assay. The apoptosis signal in the vwa1 + p53 MO group did not coincide with EGFP fluorescence. Arrows: pharyngeal arches. (C) Comparison of apoptotic and proliferative numbers of neural crest cells in pharyngeal arches between vwa1 + p53 MO-injected embryos and uninjected control embryos. The difference in cell proliferation was statistically significant (p < 0.05, Student’s t-test). Lower/upper whiskers: 1^st and 4^th quartiles of the data; box: 2^nd and 3^rd quartiles of the data; mid-line: median of the data; ×: mean value of the data; *: significantly different.

Expression Data

Gene:	EGFP
Fish:	ba2Tg ba2Tg + MO1-vwa1 + MO4-tp53
Knockdown Reagents:	MO1-vwa1 MO4-tp53
Anatomical Term:	neural crest
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ioz16Tg + MO1-vwa1 + MO4-tp53
Knockdown Reagents:	MO1-vwa1 MO4-tp53
Observed In:	neural crest cell apoptotic process neural crest cell cell growth
Stage:	Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol