FIGURE

Figure 3

ID

ZDB-FIG-200716-3

Publication

Gans et al., 2020 - Klf9 is a key feedforward regulator of the transcriptomic response to glucocorticoid receptor activity

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Figure 3

CRISPR-Cas9-mediated disruption of zebrafish klf9. (A) Schematic of the klf9 locus and encoded protein showing location of the gRNA target in exon 1, which introduces a premature stop codon upstream of the DNA binding domain. (B) Sequence of klf9 exon 1, with 5′ untranslated region in small case and coding sequence in upper case. The location of the 2 base pair deletion generated by CRISPR is shown in gray font; the gRNA target sequence is underlined; and the premature stop codon introduced by frameshift is noted with an asterisk. (C) Effect of chronic cortisol treatment and the frameshift mutation on relative levels of klf9 mRNA and pre-mRNA, measured by qRT-PCR. The results show the averages and SEM of three biological replicates; significance values were obtained by ANOVA.

Expression Data

Gene:	klf9
Fish:	AB klf9^mdi5/mdi5 (AB)
Condition:	chemical treatment: Hydrocortisone Sodium Succinate
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB klf9^mdi5/mdi5 (AB)
Condition:	chemical treatment: Hydrocortisone Sodium Succinate
Observed In:	whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

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