ZFIN Image: Gans et al., 2020, Figure 3

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Figure Caption

Figure 3

CRISPR-Cas9-mediated disruption of zebrafish klf9. (A) Schematic of the klf9 locus and encoded protein showing location of the gRNA target in exon 1, which introduces a premature stop codon upstream of the DNA binding domain. (B) Sequence of klf9 exon 1, with 5′ untranslated region in small case and coding sequence in upper case. The location of the 2 base pair deletion generated by CRISPR is shown in gray font; the gRNA target sequence is underlined; and the premature stop codon introduced by frameshift is noted with an asterisk. (C) Effect of chronic cortisol treatment and the frameshift mutation on relative levels of klf9 mRNA and pre-mRNA, measured by qRT-PCR. The results show the averages and SEM of three biological replicates; significance values were obtained by ANOVA.

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Acknowledgments

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