FIGURE

Figure 5

ID

ZDB-FIG-200627-6

Publication

Weaver et al., 2020 - Hyaloid vasculature and mmp2 activity play a role during optic fissure fusion in zebrafish

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Figure 5

Hyaloid vasculature is a source of mmp2 during optic fissure fusion. (A) Fluorescent whole mount in situ hybridization comparing mmp2, mmp14a and mmp14b expression in WT vs pax2a^−/− and DMSO vs DMH4 treated embryos at 32 hpf. Broken white lines outline the OF retinal lobes. mmp2, 14a and 14b expression within the OF is reduced in pax2a^−/− and DMH4 treated embryos. Scale bar = 50 μm. (B) qPCR analysis confirms a decrease in expression of mmp2, 14a and 14b in both pax2a^−/− and DMH4 treated embryos. (C) Two color whole mount in situ hybridization simultaneously examining mmp2 and kdrl, or mmp2 and rorB expression at 32 hpf. DNA was stained with DAPI. Scale bar = 50 μm. Clear overlap of signal is observed for mmp2 and kdrl, but not mmp2 and rorB.

Expression Data

Genes:	kdrl mmp2 mmp14a mmp14b rorb
Fish:	WT pax2a^tu29a/tu29a
Condition:	chemical treatment by environment: vascular endothelial growth factor receptor antagonist
Anatomical Terms:	optic furrow retina
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT pax2a^tu29a/tu29a
Condition:	chemical treatment by environment: vascular endothelial growth factor receptor antagonist
Observed In:	optic furrow
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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