Fig. s6

CRISPR/Cas9 injection per se does not affect HIF signalling. A-D. Representative pictures of 5 dpf CRISPANT mutants created by redundantly targeting lamb1b gene via co-injection of 4x gRNAs in vhl+/-(phd3:eGFP) incross-derived embryos (n = 400). Uninjected embryos were used as control (n = 470). Fluorescence, exposure = 991,4 ms. Scale bar 500 μm. E. Statistical analysis performed on mean grey values quantification (at the level of the head, liver and tail), after phenotypic analysis on 5 dpf lamb1b 4x gRNAs injected and uninjected vhl+/-(phd3:eGFP) incross-derived larvae. vhl-/- uninjected n = 24 larvae: head 54.83 ± 3.68 (mean ± s.e.m); liver 77.86 ± 6.46 (mean ± s.e.m); tail 19.56 ± 1.43 (mean ± s.e.m). vhl-/- injected n = 25 larvae: head 59.74 ± 4.05 (mean ± s.e.m); liver 83.23 ± 5.92 (mean ± s.e.m); tail 19.9 ± 1.38 (mean ± s.e.m); unpaired t-test (all panels: *P < 0.05; **P < 0.01; ***P <0.001; ****P < 0.0001).