FIGURE

Fig. 8

ID

ZDB-FIG-200515-16

Publication

Rothschild et al., 2020 - Genetic compensation of γ CaMKII, an evolutionarily conserved gene

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Fig. 8

Morphological defects in camk2g1 CRISPR embryos. 4 gRNAs were designed against camk2g1 to generate G0 camk2g1 CRISPR embryos (A). G0 camk2g1 CRISPR embryos show variable phenotypes with shortened anterior posterior axis and tail curvatures evident at 48hpf (C) compared to control embryos (B). Kidney cysts were absent in control embryos (D) and developed by 48hpf in CRISPR embryos (E, asterisks). Otoliths were smaller in camk2g1 CRISPR embryos compared to controls (F, G). qPCR of CaMKII genes in camk2g1 CRISPR embryos at 24hpf relative to ef1α (H). Dorsal, anterior to the left (D, E). Lateral, anterior to the left (F, G). * p < 0.05.

Expression Data

Genes:	camk2a camk2b1 camk2g1
Fish:	WIK/AB + CRISPR1-camk2g1 + CRISPR5-camk2g1 + CRISPR6-camk2g1 + CRISPR7-camk2g1
Knockdown Reagents:	CRISPR1-camk2g1 CRISPR5-camk2g1 CRISPR6-camk2g1 CRISPR7-camk2g1
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WIK/AB + CRISPR1-camk2g1 + CRISPR5-camk2g1 + CRISPR6-camk2g1 + CRISPR7-camk2g1
Knockdown Reagents:	CRISPR1-camk2g1 CRISPR5-camk2g1 CRISPR6-camk2g1 CRISPR7-camk2g1
Observed In:	kidney otolith post-vent region whole organism whole organism dorsal-ventral axis
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Gene, 742, Rothschild, S.C., Ingram, S.R., Lu, F.I., Thisse, B., Thisse, C., Parkerson, J.A., Tombes, R.M., Genetic compensation of γ CaMKII, an evolutionarily conserved gene, 144567, Copyright (2020) with permission from Elsevier. Full text @ Gene