FIGURE

Fig. 6

ID

ZDB-FIG-200426-21

Publication

Zhang et al., 2020 - Wnt-PLC-IP₃-Connexin-Ca²⁺ axis maintains ependymal motile cilia in zebrafish spinal cord

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

<italic>Cx43</italic> null mice have reduced motile cilia in ECs.

a, b The SCs of P1 pups with the indicated Cx43 genotype were cross-sectioned, double immunostained with anti-Cx43 and anti-ARL13B antibodies (a) or anti-Phospho-Cx43 (Ser386) antibody (b), and counterstained with DAPI. Asterisks indicate the central canal (CC). Images are oriented ventral to the bottom. Scale bars = 20 μm. c, d Quantifications of motile cilia per section in embryos in a and Cx43 signals per section in embyos in b. Mean ± SD. ***P < 0.001 and ****P < 0.0001 by one-way ANOVA with Tukey’s HSD post hoc test (n = 3 mice per group; one frame per mouse). ns, not significant. e TEM of the SCs of P1 pups with the indicated Cx43 genotype. Arrowheads indicate cross-sectioned motile cilia protruding to the CC and arrow marks a cilium-like structure embedded in an EC. Regions marked by arrowheads or arrow are magnified in the respective lower panels. f The human spinal cord at C1 level from a cadaver was stained with anti-Cx43 and anti-acetylated-α-tubulin antibodies. The boxed areas are magnified in the respective lower panels. Arrowhead denotes staining of Cx43 and arrow motile cilia. Scale bar = 100 μm. g A proposed model for the maintenance of ependymal motile cilia by the Wnt-PLC-IP₃-Connexin-Ca²⁺ axis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.