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Knockdown of <italic>cspg4</italic> affects paraxial mesoderm marker <italic>myoD</italic> and notochord marker <italic>ntl</italic> expression pattern.(A) Cspg4 morphants had a wider and shorter (mild) or unilateral (severe) myoD expression pattern compared to control morphants (normal) at 12 hpf. (B) The notochord was wider and shorter (mild) or absence (severe) in cspg4 morphants compared to control morphants (normal) at 12 hpf. (C) The percentage of embryos with abnormal myoD expression pattern in each group (control, n = 41; cspg4 knockdown, n = 40; cspg4 rescue, n = 28; wnt11 rescue, n = 24). (D) The percentage of embryos with abnormal ntl expression pattern in each group (control, n = 73; cspg4 knockdown, n = 43; cspg4 rescue, n = 67; wnt11 rescue, n = 35). The significant difference of normal rate was tested using Kruskal–Wallis test followed by Dunn’s multiple comparisons test. Different letters (a, b or c) indicate significant difference of normal rate between groups (p ≤ 0.05).
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