slc25a46238s zebrafish are more resilient to slc25a46 morpholino (MO) injections than WT controls: (A) Confocal micrographs of 48 hpf zebrafish motoneurons stained with znp1 (cyan), whole mount, Z stack, lateral view captured above the yolk extension. Scale bar = 50 um. (B) Qualitative assessment of the motoneuron axon phenotypes: normal represent stereotypical “hook-like” axon path as in control images; disrupted–any number of abnormal motoneuron axons, such as axonal projections crossing into a nearby segment, truncated axons with projections not reaching back up to form the hook shape or aberrant hooks missing stereotypical branching pattern (indicated by white asterisks). P-values for comparisons of phenotypes between genotypes is calculated by Fisher’s exact test (* for p<0.05; *** for p<0.001). N represents the number of individual larvae with observed motoneuron phenotype. Error bars represent SEM. (C) Brightfield micrographs of 48 hpf zebrafish larvae, lateral view. Images represent the most commonly observed phenotype within a group. Scale bar = 0.5 mm. Red arrows indicate smaller eyes, eye coloboma, shorter trunk in phenotypically distinct zebrafish.
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