Fig 1

Genome editing of slc25a46 gene in zebrafish using CRISPR/Cas9 system: (A) Schematic diagram of zebrafish slc25a46 gene with a frameshift in exon 8 indicated as a red box and a premature stop codon at amino acid position 238 (allele slc25a46^238s). The magenta arrows indicate five CRISPR guides injected together to create both F0 crispants and stable mutants. (B) Fragment analysis of the amplified CRISPR targeted region from the FAM-labeled PCR product (365 base pair amplicon) showing WT peak in control and multiple peaks indicating insertions and deletions (indels) in a representative slc25a46 F0 sample. X-axis represents base pair number; Y-axis represents signal intensity. (C) Sequence traces of wild-type (WT) and slc25a46^238s stable mutant zebrafish: exon 8, residues 223–238 indicating homozygous frameshift (in red); deletions are indicated with a triangle, insertion is underlined.