FIGURE

Fig. 2

ID

ZDB-FIG-200406-267

Publication

Nagarajan et al., 2019 - CNS myelin protein 36K regulates oligodendrocyte differentiation through Notch

Other Figures

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Fig. 2

Translation blocking MOs specifically knock down 36K protein expression. (a) Anti‐36K Western blot on whole larvae tissue showing 36K MO1 knockdown and rescue at 4 dpf. (b) Lateral view of control MO and MO1 injected zfl at 4 dpf. Scale bars: 500 μm. (c) Reduction in body length of the MO larvae at 3, 4, and 5 dpf. Sample sizes: N = 3; n shown in each bar. Two way ANOVA p ≤ .0001 (****) followed by Tukey's multiple comparisons test for control MO versus MO1 and control MO versus MO2. MO1 versus MO1 + RNA was always significantly different (not shown). Control MO versus MO1 + RNA was not significant at any of the three time‐points (p > .05). (d) Zebrafish overview sketch highlighting different CNS regions: shown as red box (e, hindbrain) and as green box (f, spinal cord). (e,f) Comparable sagittal paraffin sections counter stained with hematoxylin depicting anti‐36K staining in the hindbrain (e) and spinal cord (f) at 4 dpf in control MO (top; intense staining) and MO1 (bottom; reduced/weak staining) zfl. Scale bars: 50 μm

Expression Data

Gene:	dhrs12la
Antibody:	Ab1-dhrs12la
Fish:	WT WT + MO1-dhrs12la
Knockdown Reagent:	MO1-dhrs12la
Anatomical Terms:	hindbrain spinal cord whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dhrs12la WT + MO2-dhrs12la
Knockdown Reagents:	MO1-dhrs12la MO2-dhrs12la
Observed In:	hindbrain spinal cord whole organism
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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