FIGURE

Figure 10.

ID

ZDB-FIG-200406-235

Publication

García-González et al., 2020 - Identification of slit3 as a locus affecting nicotine preference in zebrafish and human smoking behaviour

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Figure 10.

Zebrafish breeding scheme to generate F<sub>3</sub> families.

F₂ ENU-mutagenized zebrafish, heterozygous for multiple mutations across the entire genome were obtained from the Wellcome Sanger Institute as part of the Zebrafish Mutation Project. At QMUL, heterozygous F₂ fish were incrossed to generate 30 F₃ families, each containing 10–20 nonsense or essential splice site mutations and about 1500 additional exonic and intronic point mutations. F₃ Families were arbitrarily numbered 1–30. Expected mutation rate and type of mutations in coding regions are specified on the right hand side.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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