Co-injection of drl and lrrc15 mRNA rescues hemato-vascular development in jam3bsa37 embryos. (A) Expression of runx1, fli1a and gata1a in jam3bsa37 embryos uninjected or injected with drl mRNA, lrrc15 mRNA, or both drl and lrrc15 mRNA. (B) Representative image (left) and quantification (right) of cd41:GFP; kdrl:mCherry double-positive cells in jam3bsa37 embryos uninjected or co-injected with drl and lrrc15 mRNA. White arrowheads indicate double-positive cells in the ventral floor of the DA in jam3bsa37 embryos injected with both drl and lrrc15 mRNA. Magnification of boxed area shown underneath. Data are mean±s.d. *P<0.001 (unpaired two-tailed Student's t-test). (C) Expression of rag1 in the thymus in jam3bsa37 embryos uninjected or co-injected with drl and lrrc15 mRNA. Percent distribution of runx1 expression in A is shown in Fig. S8A. Black arrowheads indicate the expression domain of each gene. Numbers in bottom right of panels indicate the number of embryos showing the displayed expression pattern over the total number of analyzed embryos. Scale bars: 100 μm.
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