The phenotype of age-related transcriptional changes detected in old wildtype animals is partially mimicked by Grna and Grnb deficiency in young mutant animals. (a,b) Heat maps depicting rlog-transformed values of the top 100 significantly upregulated and downregulated genes in old wildtype telencephali (young wildtype were used as a reference) in young wildtype animals, old wildtype animals, young mutant animals, old mutant animals, young wildtype Mpeg1+ cells, and young mutant Mpeg1+ cells. Green boxes: DEGs in old wildtype animals with the same direction of regulation in young mutant and old mutant animals, but not regulated in mutant microglial Mpeg1+ cells. Red boxes: DEGs in old wildtype animals with comparable levels in young mutant and old mutant animals, regulated in the same direction in mutant microglial Mpeg1+ cells. Black boxes: DEGs in old wildtype animals with comparable values in young mutant and old mutant animals, regulated in the opposite direction in mutant microglial Mpeg1+ cells. (c,d) Venn diagrams depicting common DEGs in old wildtype and young mutant telencephali (young wildtype were used as a reference). (e,f) Histograms depicting gene ontology (GO) terms enriched in the set of common upregulated and downregulated genes in old wildtype and young mutant telencephali (young wildtype were used as a reference).
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