FIGURE

Fig. 3

ID

ZDB-FIG-200319-7

Publication

Marín-Juez et al., 2019 - Coronary Revascularization During Heart Regeneration Is Regulated by Epicardial and Endocardial Cues and Forms a Scaffold for Cardiomyocyte Repopulation

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Fig. 3

Epicardial Hypoxia Stimulates Epicardial cxcl12b Expression and Coronary EC Proliferation via Hif1a (A) qPCR analysis of aldh1a2 and egln3 mRNA levels in sorted tcf21:RFP+ cells from 7 dps and 7 dpci ventricles (n = 4). (B and C) 7 dps (B) and 7 dpci (C) ventricles stained for epicardium (green), hypoxyprobe (red), and DNA (blue). Insets show high-magnification images of sham (B) and regenerating (C) epicardium. (D) qPCR analysis of cxcl12b mRNA levels in WT ventricles at 7 dps (n = 4) and 7 dpci (n = 4) and in hif1a−/− ventricles at 7 dpci (n = 4). (E and F) WT (E) and hif1a−/− (F) ventricles stained for endothelial nuclei (red), PCNA (green), and DNA (blue). Insets show high-magnification images of proliferating cECs (white arrowheads). (G–I) cEC proliferation in WT (n = 4), and hif1a−/− (n = 4) ventricles at 96 hpci (G). Ventricles from PBS- (H) and DMOG- (I) treated animals stained for endothelial nuclei (red), PCNA (green), and DNA (blue). Insets show high-magnification images of proliferating cECs (white arrowheads). (J) cEC proliferation in PBS- (n = 4), and DMOG- (n = 4) treated ventricles at 96 hpci. White dotted lines delineate the injured area. Data in graphs expressed as mean ± SEM. ns, no significant difference, ∗p < 0.05, ∗∗p < 0.01. Scale bars: 100 μm.

Expression Data

Genes:	aldh1a2 cxcl12b egln3
Fish:	WT pd37Tg hif1ab^bns90/bns90; hif1aa^bns89/bns89
Condition:	cryoablation: heart
Anatomical Terms:	regenerating tissue cardiac ventricle regenerating tissue epicardium
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT pd37Tg hif1ab^bns90/bns90; hif1aa^bns89/bns89
Conditions:	cryoablation: heart chemical treatment by injection: dimethyloxalylglycine, cryoablation: heart
Observed In:	blood vessel endothelial cell cell population proliferation regenerating tissue cardiac ventricle regenerating tissue cell population proliferation regenerating tissue epicardium
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 51, Marín-Juez, R., El-Sammak, H., Helker, C.S.M., Kamezaki, A., Mullapuli, S.T., Bibli, S.I., Foglia, M.J., Fleming, I., Poss, K.D., Stainier, D.Y.R., Coronary Revascularization During Heart Regeneration Is Regulated by Epicardial and Endocardial Cues and Forms a Scaffold for Cardiomyocyte Repopulation, 503-515.e4, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell