FIGURE

Fig. 6

ID

ZDB-FIG-200306-109

Publication

Asakawa et al., 2020 - Optogenetic modulation of TDP-43 oligomerization accelerates ALS-related pathologies in the spinal motor neurons

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Fig. 6

Long-term illumination induces opTDP-43h aggregation that seeds non-optogenetic TDP-43 aggregation.

a Chronic field illumination of unrestrained Tg[mnr2b-hs:EGFP-TDP43z] Tg[mnr2b-hs:opTDP-43h] fish by blue LED light. b Live imaging of the spinal motor column from 48 to 120 hpf. Horizontal dashed lines demarcate approximate positions of dorsal and ventral limits of the spinal cord. c Cytoplasmic opTDP-43h foci colocalize with EGFP-TDP-43z. d FRAP analyses of nuclear opTDP43h that had not been exposed to blue light (left, Dark Pre) and cytoplasmic opTDP43h foci that had been induced by a 72-h blue light illumination (right, BL Pre). Bleaching was performed at 120 hpf. Yellow dashed circles (Pre) include photobleached area and arrows indicate the bleached position. e Quantification of fluorescent recovery. The results were obtained from 6 cells in independent 6 animals for each condition. Error bars show SD, and the center of the error bars is the average value. The bars indicate 20 µm (b), 5 µm (c, d). Error bars show SD.

Expression Data

Genes:	EGFP mRFP
Fish:	nkmnr2bhsegftdp43zTg; nkmnr2bhsoptdp43hTg
Condition:	visible light
Anatomical Terms:	CaP motoneuron cytoplasm CaP motoneuron nucleus spinal cord motor neuron
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nkmnr2bhsegftdp43zTg; nkmnr2bhsoptdp43hTg
Condition:	visible light
Observed In:	spinal cord motor neuron
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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