ZFIN Image: Asakawa et al., 2020, Fig. 6

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Figure Caption

Fig. 6 Long-term illumination induces opTDP-43h aggregation that seeds non-optogenetic TDP-43 aggregation.

a Chronic field illumination of unrestrained Tg[mnr2b-hs:EGFP-TDP43z] Tg[mnr2b-hs:opTDP-43h] fish by blue LED light. b Live imaging of the spinal motor column from 48 to 120 hpf. Horizontal dashed lines demarcate approximate positions of dorsal and ventral limits of the spinal cord. c Cytoplasmic opTDP-43h foci colocalize with EGFP-TDP-43z. d FRAP analyses of nuclear opTDP43h that had not been exposed to blue light (left, Dark Pre) and cytoplasmic opTDP43h foci that had been induced by a 72-h blue light illumination (right, BL Pre). Bleaching was performed at 120 hpf. Yellow dashed circles (Pre) include photobleached area and arrows indicate the bleached position. e Quantification of fluorescent recovery. The results were obtained from 6 cells in independent 6 animals for each condition. Error bars show SD, and the center of the error bars is the average value. The bars indicate 20 µm (b), 5 µm (c, d). Error bars show SD.

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Acknowledgments

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