Fig. 7

Gastrosomal Defects Affect Microglial Functionality

(A) 4 dpf wild-type Tg(mpeg:GFP-caax) microglia stained with Nile Blue (cyan) in an embryo treated with Camptothecin to increase apoptosis. Scale bar, 5 μm. The white dotted line marks the gastrosome.

(B) SPIM time-lapse of representative 4 dpf wild-type Tg(mpeg:GFP-caax) microglia stained with Nile Blue (cyan) in an embryo treated with Camptothecin to increase apoptosis. Scale bar, 10 μm. The white dotted line marks the shrinking gastrosome. See also Video S8.

(C) Quantification of microglia responding to an injury in wild-type and blb^NY007 microglia at different developmental stages; wt, 3.5 dpf, n = 20; blb^NY007, 3.5 dpf, n = 20; wt, 4 dpf, n = 20; and blb^NY007, 4 dpf, n = 16; whiskers: 5th/95th percentile, p value < 0.0001. See also Video S9 (left, wild-type embryo and right, blb embryo).

(D) Quantification of engulfment duration in wild-type (n = 20) and blb^NY007 microglia (n = 11), whiskers: 5th/95th percentile, p value < 0.001.

(E) RAW264.7 macrophages unfed (left column) or fed with HK H2B mCherry cells and fixed 24 h after apoptosis induction (right column). Slc37a2 antibody staining in untreated controls (upper panels) and in cells transfected with an siRNA targeting Slc37a2.

(F) Quantification of the gastrosomal size (μm) in RAW macrophages fed with apoptotic HeLa cells in the absence or in the presence of Slc37a2 siRNA, p value < 0.0001. Representative images of this experiment in Figure S3A (yellow arrowheads).