FIGURE

Fig. 6

ID

ZDB-FIG-191224-5

Publication

Villani et al., 2019 - Clearance by Microglia Depends on Packaging of Phagosomes into a Unique Cellular Compartment

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Fig. 6

In Macrophages and Microglia, the Gastrosome Contains Lipid and Membranes

(A) SPIM time-lapse of a representative 3 dpf wild-type Tg(mpeg:GFP-caax) microglia in an embryo treated with Camptothecin to increase apoptosis. Scale bar 5 μm. The while dot marks the growing compartment. See also Video S6.

(B–E) RAW264.7 macrophages fixed 9 h after apoptosis induction of unlabeled HeLa cells. Stained with WGA (B), Nile Blue (C), Bodipy (D), and Nile Red (E). The white arrow points to the gastrosome. Scale bar, 5 μm.

(F–I) 4 dpf blb microglia stained with WGA (F), Nile Blue (G), Bodipy (H) and Nile Red (I). Scale bar, 10 μm.

Expression Data

Gene:	EGFP
Fish:	zh901Tg
Condition:	chemical treatment by environment: camptothecin
Anatomical Terms:	microglial cell phagocytic vesicle microglial cell plasma membrane
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zh901Tg
Condition:	chemical treatment by environment: camptothecin
Observed In:	brain apoptotic process microglial cell phagocytic vesicle
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 49(1), Villani, A., Benjaminsen, J., Moritz, C., Henke, K., Hartmann, J., Norlin, N., Richter, K., Schieber, N.L., Franke, T., Schwab, Y., Peri, F., Clearance by Microglia Depends on Packaging of Phagosomes into a Unique Cellular Compartment, 77-88.e7, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell