FIGURE

Fig. 5

ID

ZDB-FIG-190923-3

Publication

Wang et al., 2019 - A long noncoding RNA cluster-based genomic locus maintains proper development and visual function

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Fig. 5

CNIB1 influences visual function in zebrafish. (A) Representative eye images of the zebrafish offspring at 5 dpf from CNIB1^+/+, CNIB1^+/− 1, and CNIB1^+/− 2 incross. Scale bars: 300 μm. (B) The schematic representation of the eye diameters gathered from figure 5A. CNIB1^+/+, n = 19, CNIB1^+/− 1, n = 13, CNIB1^+/− 2, n = 13. (C) Schematic representation of the zebrafish larval optokinetic response (OKR) experiment. (D) The ratio of eye velocity to the angular velocity of the grating (gain value) assayed by OKR in zebrafish offspring at 5 dpf from CNIB1^+/+ (n = 9), CNIB1^+/− 1 (n = 7), and CNIB1^+/− 2 (n = 7) incross. (E) Representative immunofluorescence staining images of the Th positive (Th⁺) DA neurons shown in the retinas of zebrafish offspring at 5 dpf from CNIB1^+/+ and CNIB1^+/− 2 incross. Scale bars: 50 μm. (F) The schematic representation of E. CNIB1^+/+, n = 15; CNIB1^+/− 2, n = 24. (G) The expression levels of th detected by RT-qPCR in zebrafish offspring from CNIB1^+/+ (n = 30) and CNIB1^+/− 2 (n = 30) incross, normalized to β-actin. (H) The effects of CNIB1 lncRNAs on the OKR assay in the indicated zebrafish offspring. Control-injected CNIB1^+/+, n = 6; CNIB1_S-injected CNIB1^+/+, n = 6; CNIB1_AS-injected CNIB1^+/+, n = 6; Control-injected CNIB1^+/− 2, n = 6; CNIB1_S-injected CNIB1^+/− 2, n = 6; CNIB1_AS-injected CNIB1^+/− 2, n = 6.

Expression Data

Antibody:	Ab3-th
Fish:	AB lnc.cnib1^zf3099/+
Anatomical Term:	retina dopaminergic neuron
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lnc.cnib1^zf3098/+ lnc.cnib1^zf3099/+
Observed In:	eye optokinetic behavior retina dopaminergic neuron
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.