FIGURE

Fig. 2

ID

ZDB-FIG-190919-29

Publication

Shainer et al., 2019 - Agouti-Related Protein 2 Is a New Player in the Teleost Stress Response System

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Fig. 2

Zebrafish AgRP1 Neurons Do Not Co-express NPY or Gad1b

(A) In situ hybridization (ISH) analysis of NPY in 5-dpf larva, lateral view; eyes were dissected. NPY is expressed in the stratum periventriculare layer of the optic tectum and in the pallium but is not detected in the hypothalamus. H, hypothalamus; P, pallium; SPV, stratum periventriculare.

(B) ISH analysis of NPY in 5-dpf larva, dorsal view; eyes were dissected. NPY is expressed in the stratum periventriculare layer of the optic tectum and in the pallium.

(C) Hypothalamus of 5-dpf agrp1:Gal4-VP16; UAS:nfsB-mCherry, gad1b:EGFP. The EGFP is not localized to AgRP1 neurons (arrowheads). Scale bar represents 10 μm. See also Video S1.

(D) Same as (C), presentation of the EGFP layer only. The localization of the AgRP1 neurons is marked by arrowheads to demonstrate the absence of EGFP.

Expression Data

Genes:	GFP mCherry npy
Fish:	WT c264Tg; nns25Tg; tlv04Tg
Anatomical Terms:	dorsal telencephalon hypothalamus GABAergic neuron hypothalamus neuron periventricular grey zone
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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