ZFIN Image: Shainer et al., 2019, Fig. 2

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Figure Caption

Fig. 2

Zebrafish AgRP1 Neurons Do Not Co-express NPY or Gad1b

(A) In situ hybridization (ISH) analysis of NPY in 5-dpf larva, lateral view; eyes were dissected. NPY is expressed in the stratum periventriculare layer of the optic tectum and in the pallium but is not detected in the hypothalamus. H, hypothalamus; P, pallium; SPV, stratum periventriculare.

(B) ISH analysis of NPY in 5-dpf larva, dorsal view; eyes were dissected. NPY is expressed in the stratum periventriculare layer of the optic tectum and in the pallium.

(C) Hypothalamus of 5-dpf agrp1:Gal4-VP16; UAS:nfsB-mCherry, gad1b:EGFP. The EGFP is not localized to AgRP1 neurons (arrowheads). Scale bar represents 10 μm. See also Video S1.

(D) Same as (C), presentation of the EGFP layer only. The localization of the AgRP1 neurons is marked by arrowheads to demonstrate the absence of EGFP.

Figure Data

Expression / Labeling details

Acknowledgments

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