Fig. 1

Sulfatases knockdown induces circulatory malformations. (a) Fluorescence images of a WT zebrafish larvae at 48 hpf after injection of tetramethylrhodamine labeled dextran (MW 2000kDa) in the cardinal vein. CTRL-MO injected larvae exhibit regular development of the tail circulation.  larvae display minimal blood circulation in the tail and potential premature shunting between the caudal artery and the caudal vein.  larvae display minor disruptions of the tail vasculature.  larvae exhibit a minor injury to the tail vasculature including reduced circulation in the distal tail areas. (b) Fluorescence images of Tg(flk1-mCherry) larvae at 72 hpf. CTRL-MO injected larvae display regular development of the tail circulation.  larvae display a discontinuation of the caudal artery in the tail vasculature indicative of an arteriovenous shunt between caudal artery and caudal vein. The blood circulation in the distal tail is diminished.  larvae exhibit disruptions of the tail vasculature and reduced blood circulation in the distal tail.  larvae exhibit signs of vascular injury in the tail including reduced circulation in the distal tail areas.