FIGURE

Fig. 3

ID

ZDB-FIG-190913-59

Publication

Frikstad et al., 2019 - A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling

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Fig. 3

Ciliary Localization of CSPP-L at Motile and Primary Cilia

(A) CSPP-L detection by post-embedding IEM of mouse tracheal epithelial cells. Panels depict close ups of (i) cilia tips, (ii and iii) basal bodies, (iv) cilia axonemes, and (v) apically localized electron-dense particles.

(B and C) IFM of mouse tracheal epithelial cells showing axonemal MTs (glutamylated tubulin, red) and CSPP-L (B, green) or Sentan (C, green). Right panels show magnifications of indicated regions.

(D and E) 3D-SIM IFM of hTERT-RPE1 cells expressing monomeric NeonGreen (mNG)-CSPP-L and co-stained for centrosomal marker γ-tubulin (white) and cilia membrane marker ARL13B (red). Scale bars in magnified areas, 500 nm.

mNG-CSPP-L decorates axonemal MTs throughout the transition zone and concentrates at the tip (D and E). Centriolar satellite localization is frequently found and exemplified in (E) and Figure S4C.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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