FIGURE

Fig. 6

ID

ZDB-FIG-190820-9

Publication

Fontenas et al., 2019 - The Neuromodulator Adenosine Regulates Oligodendrocyte Migration at Motor Exit Point Transition Zones

Other Figures

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Fig. 6

Peripheral OPCs Myelinate Spinal Motor Axons in gpr126Mutant Larvae

(A) wif1 mRNA expression in WT and gpr126^−/− larvae at 3 dpf shows MEP glia (arrowheads). SC, spinal cord; N, notochord.

(B) Live images of photoconverted sox10:eos larvae at 3 dpf show Eos⁺MEP glia (arrowheads) in both WT and gpr126^−/− larvae.

(C) Mean ± SEM of peripheral OPCs in 3 dpf gpr126^−/− larvae treated with DMSO or SCH-58261 from 36 hpf to 3 dpf. n = 8 DMSO, n = 6 SCH-58261. ^∗∗p = 0.009.

(D) Frames from a 15 h time-lapse movie of a olig2:dsred;nkx2.2a:megfp;gpr126^−/− larva treated with SCH-58261 from 36 hpf to 3 dpf; 0′ is 57 hpf. Arrowheads mark a nkx2.2a⁺/olig2⁺ OPC ensheathing motor axons.

(E) Images of MBP antibody (arrowheads) on peripheral nerves of 4 dpf olig2:dsred;gpr126^−/− larvae treated with DMSO or SCH-58261 from 36 hpf to 3 dpf. Asterisk marks peripheral OPC.

(F) Live images of 4 dpf WT and gpr126^−/−;olig2:dsred;mbp:egfp larvae treated with DMSO or SCH-58261 from 36 hpf to 3 dpf. Asterisk marks olig2⁺ peripheral OPC with mbp⁺ membrane sheaths (arrowheads) around peripheral motor axons. Scale bars, 20 μm.

Expression Data

Genes:	DsRed2 EGFP wif1
Antibody:	Ab1-mbp
Fish:	AB adgrg6^st49/st49 adgrg6^st49/st49; ue2Tg; vu19Tg adgrg6^st49/st49; vu19Tg
Condition:	chemical treatment by environment: adenosine A2A receptor antagonist
Anatomical Terms:	oligodendrocyte peripheral nervous system oligodendrocyte peripheral neuron myelin sheath spinal nerve root glial cell
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	adgrg6^st49/st49; ue2Tg; vu19Tg adgrg6^st49/st49; vu19Tg
Condition:	chemical treatment by environment: adenosine A2A receptor antagonist
Observed In:	motor neuron axon oligodendrocyte peripheral nervous system peripheral nervous system oligodendrocyte peripheral neuron myelin sheath peripheral neuron myelination
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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