ZFIN Image: Fontenas et al., 2019, Fig. 6

Image

Figure Caption

Fig. 6

Peripheral OPCs Myelinate Spinal Motor Axons in gpr126Mutant Larvae

(A) wif1 mRNA expression in WT and gpr126^−/− larvae at 3 dpf shows MEP glia (arrowheads). SC, spinal cord; N, notochord.

(B) Live images of photoconverted sox10:eos larvae at 3 dpf show Eos⁺MEP glia (arrowheads) in both WT and gpr126^−/− larvae.

(C) Mean ± SEM of peripheral OPCs in 3 dpf gpr126^−/− larvae treated with DMSO or SCH-58261 from 36 hpf to 3 dpf. n = 8 DMSO, n = 6 SCH-58261. ^∗∗p = 0.009.

(D) Frames from a 15 h time-lapse movie of a olig2:dsred;nkx2.2a:megfp;gpr126^−/− larva treated with SCH-58261 from 36 hpf to 3 dpf; 0′ is 57 hpf. Arrowheads mark a nkx2.2a⁺/olig2⁺ OPC ensheathing motor axons.

(E) Images of MBP antibody (arrowheads) on peripheral nerves of 4 dpf olig2:dsred;gpr126^−/− larvae treated with DMSO or SCH-58261 from 36 hpf to 3 dpf. Asterisk marks peripheral OPC.

(F) Live images of 4 dpf WT and gpr126^−/−;olig2:dsred;mbp:egfp larvae treated with DMSO or SCH-58261 from 36 hpf to 3 dpf. Asterisk marks olig2⁺ peripheral OPC with mbp⁺ membrane sheaths (arrowheads) around peripheral motor axons. Scale bars, 20 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.