FIGURE

Fig. S5

ID

ZDB-FIG-190807-13

Publication

Talbot et al., 2019 - Muscle precursor cell movements in zebrafish are dynamic and require six-family genes

Other Figures

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Fig. S5

Fins are not required for MMP specification. (A-D) Brightfield images of four-dayold embryos. Brightfield images of wild-type, dnFgfrexpressing, and Δsix1a;4a;Δsix1b;4b mutant embryos. Fins are present (asterisk) in wild-type (A) and Δsix1a;4a;Δsix1b;4b mutant (B) embryos, but absent in dnFgfr-induced (C) and aldh1a2 mutant (D) embryos (aldh1a2 mutant fin phenotype previously shown by Grandel et al., 2002). (E-J) RNA in situ hybridization for (E, F) met and (G, H) lbx1a in 36 hpf embryos. (E-H) Although migratory streams are severely misshapen in aldh1a2 mutants, MMP markers lbx1a (E, F) and met (G, H) are expressed at levels comparable to wild-type embryos. In aldh1a2-/- mutant MMPs, met expression appears darker than in WT (E, F); this may be due to greater cell density in the mutant, which has impaired migration. Embryos in A-C and E-H were PTU-treated to inhibit pigment formation. MMP arrowheads are color coded as described in Figures 1 and S1. Scale bars are 100 μm.

Expression Data

Genes:	lbx1a met
Fish:	WT aldh1a2^i26/i26
Anatomical Terms:	anterior migratory muscle precursor stream middle migratory muscle precursor stream posterior migratory muscle precursor stream
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	aldh1a2^i26/i26; cz3327Tg; oz5Tg cz3327Tg; oz5Tg; pd1Tg/pd1Tg Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; cz3327Tg; oz5Tg
Observed In:	pectoral fin
Stage:	Day 4

Phenotype Detail

Acknowledgments

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