FIGURE

Fig 4

ID

ZDB-FIG-190723-826

Publication

Shim et al., 2019 - Pathway-specific protein domains are predictive for human diseases

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Fig 4

Experimental validation of novel genes for heart development in zebrafish.

(A) Tg(flk1:EGFP) zebrafish embryos injected with morpholinos (MOs) for novel candidate genes for CAD showed morphological heart abnormalities, such as peripheral edema at 3 days post-fertilization (arrows in the left panel, scale bar = 500 μm). Zebrafish embryos normally have hearts with a left ventricle (V) and right atrium (A), whereas the embryos injected with MOs related to CAD genes exhibited either no asymmetry or reversed V and A orientation (middle panels, scale bar = 200 μm). These embryos also exhibited malformed blood vessels in the trunk (asterisks in the right panel, scale bar = 200 μm). (B) MO-injected Tg(flk1:EGFP) zebrafish embryos were counted to quantify those that exhibited heart asymmetry. (C) MO-injected Tg(flk1:EGFP) zebrafish embryos were counted to quantify those that exhibited vascular defects. Over 20 MO-injected embryos per gene were counted for each analysis (A-C).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s843Tg + MO1-atp2a2b s843Tg + MO1-slc22a2 s843Tg + MO1-tram1 s843Tg + MO3-cyp11a1.1
Knockdown Reagents:	MO1-atp2a2b MO1-slc22a2 MO1-tram1 MO3-cyp11a1.1
Observed In:	atrium cardiac ventricle determination of heart left/right asymmetry heart trunk vasculature
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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