Figure 1
- ID
- ZDB-FIG-190723-352
- Publication
- Baraban et al., 2018 - Ca 2+ activity signatures of myelin sheath formation and growth in vivo
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A. Maximum intensity projection of a 3D z-stack of the first time-point from a 21 minute-long movie of a GCaMP6s expressing oligodendrocyte. Two areas of interest indicated, top corresponding to D and bottom to C. Scale bar= 10µm. Fire LookUpTable reflects grey value range 0-65,535 (black to white). B. Maximum intensity projection of all time-points of cell shown in A. Arrowheads indicate sheaths with increased fluorescence, reflecting Ca2+ activity during the movie. Arrows point to sheaths with no increase. C, D, myelin sheaths demarcated within ROIs outlined in A, at indicated times. E. ΔF/F0 over time. Arrowheads indicate sheaths shown in corresponding colours in B, C and D. F. Schematic of time-lapse imaging experiment with interspersed imaging of cell morphology (yellow) and Ca2+ (green). G. Sample images of myelin sheath morphology (under yellow bars) with intervening periods of Ca2+ imaging (under green bar). Arrowhead points to Ca2+ transient in sheath. Scale bar=5µm. H. Distribution of Ca2+ transient frequencies of 305 sheaths, analysed in 18 animals. I. Distribution of average Ca2+ transient amplitude per sheath (187 sheaths from 18 animals). Graph shows median and 1st and 3rd quartiles. J. Distribution of average Ca2+ transient duration per sheath (187 sheaths from 18 animals). Graph shows median and 1st and 3rd quartiles. K. Correlation between amplitude and duration per individual Ca2+ transient events (448 events from 187 sheaths in 18 animals, Pearson’s Correlation Test, p=0.0006). |