FIGURE

Fig. S6

ID

ZDB-FIG-190715-12

Publication

Williams et al., 2018 - Requirement of zebrafish pcdh10a and pcdh10b in melanocyte precursor migration

Other Figures

All Figure Page

Back to All Figure Page

Fig. S6

Knockdown of pcdh10b in pcdh10a mutants restores NCC migration defect. Lateral views of double transgenic zebrafish Tg(sox10:mRFP,mitfa::GFP), membrane RFP has been changed to grayscale. (A) Control wildtype embryos injected with pcdh10b MO NCCs 28hpf. (B, C) Lateral view of pcdh10a mutants injected with pcdh10b MO NCCs at 28hpf show defects in NCC migration, including in mitfa+ melanoblasts. White arrows mark NCCs that have detached from other NCCs in the migrating streams. Red arrows mark NCCs that have aggregated within the dorsal domain. (D) Percent of total time NCCs are detached from other NCCs in pcdh10b MO alone and pcdh10a-/- injected with pcdh10b MO embryos. * denotes P value of .035. Scale bar is 30um. (E) Quantification of the duration of cell detachment events across all samples, ** denotes P value of .006 (F) Table, comparison of the number of embryos imaged and the number of detachment events that occurred across all samples.

Expression Data

Genes:	pcdh8 pcdh10a pcdh10b pcdh15a pcdh18b pcdh19
Fish:	WT pcdh10a^{co1000/co1000} WT + MO2-pcdh10a
Knockdown Reagent:	MO2-pcdh10a
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pcdh10a^{co1000/co1000} WT + MO2-pcdh10a
Knockdown Reagent:	MO2-pcdh10a
Observed In:	whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 444 Suppl 1, Williams, J.S., Hsu, J.Y., Rossi, C.C., Artinger, K.B., Requirement of zebrafish pcdh10a and pcdh10b in melanocyte precursor migration, S274-S286, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.