Bif1, a C2H2 zinc-finger protein, is expressed at an early stage and becomes restricted to the erythroid lineage during primitive erythropoiesis. (A) Representation of the bif1 gene and protein structures. NLS, nuclear localization sequence. (B) Immunofluorescence using an antibody against HA and DAPI staining on 6 hpf non-injected embryos or embryos injected with bif1-HAmRNA. The area shown at higher magnification is indicated with a dotted rectangle. (C) Time-course expression of bif1 revealed by whole-mount in situ hybridization. The arrow and the dotted rectangle represents the pronephric glomerulus and the caudal hematopoietic tissue, respectively. (D) Expression of bif1 at 12 hpf in wild type and a cloche mutant. The red arrows show the expression of bif1 that is lost in the cloche mutant. (E) Double whole-mount in situhybridization against bif1 (blue) and pax2 (orange, representing the intermediate mesoderm) at 13 hpf embryos. The area shown at higher magnification is indicated with a dotted rectangle. (F) Images of 23 hpf fluorescent fish carrying two constructs: Tg(bif1:GFP-LT) and Tg(gata1:DsRed).
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