FIGURE

Fig. S11

ID

ZDB-FIG-190711-13

Publication

Ghersi et al., 2019 - bif1, a new BMP signaling inhibitor, regulates embryonic hematopoiesis in the zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. S11

bif1 knockdown is rescued by co-injection with bif1 mRNA. A. Schematic representation of the genomic structure of bif1. The red line corresponds to the morpholino-targeted site. Dark and light blue arrows correspond to the primers amplifying a long (972 nucleotides) and a short product (224 nucleotides), respectively. The size of the intron is 1122 nucleotides. B. PCR on cDNA obtained from embryos at 24hpf, injected with MO-control or MO-bif1, or non-injected. In bif1-morphants, the wild-type band disappears, suggesting retention of the intron. C. Bright-field pictures of embryos at 3dpf stained with O-dianisidine. D. Quantification of embryos exhibiting the corresponding morphologies, dorsalization and ventralization (smaller eyes) at 3dpf. The numbers of embryos are indicated in parentheses. E. Quantification of embryos after O-dianisidine staining at 3dpf.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development