Fig. 3

A. Survival graph of ezh2(sa1199). Survival assay was performed with ezh2(sa1199) in-crossed wild type (N = 18, blue line) and mutant (N = 21, orange line) siblings until 46 dpf. Some larval lethality during raising was seen both in wild types and mutants. The graph indicates that the survival of homozygous ezh2(sa1199) mutants is not significantly different from wild type siblings. B. Pictures of wild type and mutant ezh2(sa1199) adult siblings. While wild types have normal jaws (left), adult ezh2(sa1199)mutants (right) display an open mouth phenotype (black arrowhead). C. RT-qPCR measurement of ezh1 and ezh2 mRNA in ezh2(sa1199) adults. The (decapitated) body of 70 dpf wild type (blue) and mutant (orange) individuals were lysed and the presence of ezh1 and ezh2 mRNA was quantified and normalized to the reference genes ß-actinand ef1a. The ezh1 (p = 0.9509) and ezh2 (p = 0.1493) mRNA levels were not significantly altered in mutants. D. Ezh2 protein in single 70 dpf adults and H3K27me3 in 7 dpf pooled larvae. The presence of Ezh2 was tested in wild type (N = 3, left) and mutant (N = 4, right) bodies at 70 dpf, where Ezh2 expression persists (upper panel). At 7 dpf, mutant larvae (right) show 41.8% decreased levels of the H3K27me3 mark compared to wild type (left) larvae (lower panel) and relative to β-actin loading control. For each Western blot, representative bands were cropped from the same blot and shown in boxes for clarity of presentation.