FIGURE

Fig. 5

ID

ZDB-FIG-190606-40

Publication

Pickett et al., 2018 - Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells

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Fig. 5

Mitochondrial activity depends on hair cell mechanotransduction.

(A, B) Maximum projections of hair cells from WT/Het and sputnik mutant siblings crossed to Tg[myo6b:mitoTimer]^w208. (C) mitoTimer mean fluorescence ratio for WT/Het and sputnik larvae. WT/Het: 100 ± 18.7, n = 14 fish; Mutant: 34 ± 13.1,15 mutant fish; mean (% WT/Het)±SD. Value for each fish represents the mean of 2–4 neuromasts. (D, E) Maximum projections of mitoTimer-expressing hair cells from control larvae and larvae incubated in 200 μM benzamil. (F) mitoTimer mean fluorescence ratio for larvae incubated in benzamil compared to DMSO control. Control: 100 ± 28.7; Treated: 56.6 ± 22.7, n = 17 fish per group; mean (% Control)±SD. Value for each fish represents the mean of 2–4 neuromasts. Mann-Whitney U test was used to assess significance. All larvae imaged at 5dpf. Scale bar = 5 μm.

Expression Data

Gene:	DsRed
Fish:	w208Tg cdh23^tj264/tj264; w208Tg
Condition:	chemical treatment: Benzamil
Anatomical Term:	neuromast hair cell mitochondrion
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	w208Tg cdh23^tj264/tj264; w208Tg
Condition:	chemical treatment: Benzamil
Observed In:	neuromast hair cell mitochondrion
Stage:	Day 5

Phenotype Detail

Acknowledgments

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