FIGURE

Fig. 5

ID

ZDB-FIG-190604-37

Publication

DeLaurier et al., 2019 - hdac4 mediates perichondral ossification and pharyngeal skeleton development in the zebrafish

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Fig. 5

Analysis of maternal-zygote mutant and heterozygote cartilage ossification using specimens from the hdac4^aik3 line, stained using Alcian Blue and Alizarin Red dyes.

(A) Wild-type (non-sibling) larval pharyngeal skeleton at 7 dpf, lateral view. (B) Wild-type (non-sibling) larval pharyngeal skeleton at 12 dpf, lateral view. (C–E) maternal-zygotic mutant (C and D) and heterozygote (E) pharyngeal skeletons at 7 dpf showing premature ossification of the anguloarticular bone, ventral hypohyal, and ossification of the symplectic of the hyosymplectic (indicated by asterisk), lateral views. (F) Total scores of maternal-zygotic mutants and heterozygotes for premature/excessive ossification defects. (G and H) Wild-type posterior pharyngeal arches 3 and 4 at 7 dpf (G) and 12 dpf (H), including ossification of first and second ceratobranchial cartilages at 12 dpf, indicated by arrows, ventral views. (I) Maternal-zygotic mutant showing ossification of the first and second ceratobranchial cartilages cartilages at 7 dpf, indicated by arrows, ventral view. Abbreviations: aa, anguloarticular; cb1,2, ceratobranchial 1 and 2; q, quadrate; and hhv, ventral hypohyal. A–E, Scale bar = 200 microns, G–I, Scale bar = 50 microns. Cartilage is stained blue (Alcian Blue), bone is stained red (Alizarin Red). See Fig. 2A for schematic of the pharyngeal skeleton at 7 dpf.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hdac4^aik3/aik3 (AB) hdac4^aik3/+ (AB)
Observed In:	anguloarticular ossification ceratobranchial 1 cartilage ossification ceratobranchial 2 cartilage ossification ceratohyal cartilage ossification quadrate ossification symplectic ossification ventral hypohyal bone ossification
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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Fig. 5

Fig. 5

Analysis of maternal-zygote mutant and heterozygote cartilage ossification using specimens from the hdac4aik3 line, stained using Alcian Blue and Alizarin Red dyes.

Analysis of maternal-zygote mutant and heterozygote cartilage ossification using specimens from the hdac4^aik3 line, stained using Alcian Blue and Alizarin Red dyes.