FIGURE

Fig. 1

ID

ZDB-FIG-190530-33

Publication

Chen et al., 2019 - Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology

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Fig. 1

Comparing the effects of Ddx19B or Dicer1 knockdown by Trim-Away or morpholinos in zebrafish embryos. Zebrafish embryos were co-injected with TRIM21 and antibodies (anti-Ddx19B antibody, anti-Dicer1 antibody, or control IgG) or injected with morpholinos (ddx19b-MO, Dicer1-MO, or Con-MO) at 0 hpf. a The level of Ddx19B or Dicer1 was determined by western blotting at the indicated time points. b Lateral view of eye size at the indicated time points. c Lateral view of the body axis at 2 dpf. d Eye diameter in embryos injected with different doses of TRIM21/anti-Ddx19B antibody or TRIM21/IgG was measured at the indicated time points; zebrafish numbers are indicated in brackets. e The level of miR-26a and let-7a was determined at the indicated time points. f Lateral view of embryos at the indicated time points. The data in d and eare shown as the mean ± SD of three independent experiments, *P ≤ 0.05, **P ≤ 0.01, NS indicates P > 0.05

Expression Data

Antibodies:	Ab2-dicer Ab2-zgc:193690
Fish:	AB + MO1-ddx19a AB + MO1-dicer1
Knockdown Reagents:	MO1-ddx19a MO1-dicer1
Anatomical Term:	whole organism
Stage Range:	Shield to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-ddx19a AB + MO1-dicer1
Knockdown Reagents:	MO1-ddx19a MO1-dicer1
Observed In:	eye whole organism
Stage Range:	Prim-5 to Day 5

Phenotype Detail

Acknowledgments

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