PUBLICATION

Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology

Authors
Chen, X., Liu, M., Lou, H., Lu, Y., Zhou, M.T., Ou, R., Xu, Y., Tang, K.F.
ID
ZDB-PUB-190125-2
Date
2019
Source
Genome biology   20: 19 (Journal)
Registered Authors
Keywords
Knockdown, Protein degradation, TRIM21, Trim-away
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Biotechnology/methods*
  • Embryo, Nonmammalian/metabolism
  • Proteolysis*
  • Ribonucleoproteins/metabolism*
  • Zebrafish
(all 7)
PubMed
30674345 Full text @ Genome Biol.
Abstract
Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embryos. Trim-Away depletes proteins faster than morpholinos, which enables analysis of protein function during early embryogenesis. Furthermore, Trim-Away can be applied to evaluate the role of maternally contributed proteins in zebrafish embryos. Our findings indicate that Trim-Away is a powerful tool to perform functional analysis of proteins during zebrafish development.
Genes / Markers
Marker Marker Type Name
actb1GENEactin, beta 1
ddx19aGENEDEAD-box helicase 19a
ddx19bGENEDEAD-box helicase 19b
dicer1GENEdicer 1, ribonuclease type III
mitfaGENEmelanocyte inducing transcription factor a
1 - 5 of 5
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Figures
Figure Gallery (2 images)
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Expression
No data available
Phenotype
Fish Conditions Stage Phenotype Figure
AB + MO1-ddx19acontrolPrim-5
AB + MO1-ddx19acontrolPrim-5
AB + MO1-ddx19acontrolLong-pec
AB + MO1-ddx19acontrolLong-pec
AB + MO1-ddx19acontrolProtruding-mouth
AB + MO1-ddx19acontrolDay 4
AB + MO1-ddx19acontrolDay 5
AB + MO1-dicer1controlPrim-5
AB + MO1-dicer1controlPrim-5
AB + MO1-dicer1controlLong-pec
1 - 10 of 13
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hi1464TgTransgenic Insertion
1 - 1 of 1
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Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
ddx19aMO1-ddx19aMRPHLNO
dicer1MO1-dicer1MRPHLNO
mitfaMO2-mitfaMRPHLNO
1 - 3 of 3
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Fish
Fish
AB
AB + MO1-ddx19a
AB + MO1-dicer1
AB + MO2-mitfa
1 - 4 of 4
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Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping
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Citation
Chen, X., Liu, M., Lou, H., Lu, Y., Zhou, M.T., Ou, R., Xu, Y., Tang, K.F. (2019) Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology. Genome biology. 20:19.