Fig. 8

Mcm7 knockdown in zebrafish. (A) RT-PCR of control injected (CTRL MO) and Mcm7 MO injected embryos at 24 hpf. In embryos with Mcm7 MO the original band at 340 bp partially disappeared. Instead a second band around 1500 bp could be detected, which contained intronic sequences. n = 3 independent experiments. (B) Live images of zebrafish at 48 hpf. Scale bars: 500 μm. (C) Mcm7 depleted embryos develop smaller anterior structures, which is partially rescued by co-injection of RNA encoding human MCM7. Five independent experiments. **P < 0.01, ****P < 0.0001. One-way ANOVA with Holm–Sidak's multiple comparison test. (D) Loss of Mcm2 impairs eye development. Five independent experiments. ***P < 0.001, ****P < 0.0001. One-way ANOVA with Holm–Sidak's multiple comparison test. (E) Zebrafish lacking Mcm7 develop pericardial edema. 5 independent experiments. *P < 0.05, ***P < 0.0001. One-way ANOVA with Holm–Sidak's multiple comparison test. (C–E) Numbers of embryos analysed are given below the bars in E.