ZFIN Image: Casar Tena et al., 2018, Fig. 8

Image

Figure Caption

Fig. 8

Mcm7 knockdown in zebrafish. (A) RT-PCR of control injected (CTRL MO) and Mcm7 MO injected embryos at 24 hpf. In embryos with Mcm7 MO the original band at 340 bp partially disappeared. Instead a second band around 1500 bp could be detected, which contained intronic sequences. n = 3 independent experiments. (B) Live images of zebrafish at 48 hpf. Scale bars: 500 μm. (C) Mcm7 depleted embryos develop smaller anterior structures, which is partially rescued by co-injection of RNA encoding human MCM7. Five independent experiments. **P < 0.01, ****P < 0.0001. One-way ANOVA with Holm–Sidak's multiple comparison test. (D) Loss of Mcm2 impairs eye development. Five independent experiments. ***P < 0.001, ****P < 0.0001. One-way ANOVA with Holm–Sidak's multiple comparison test. (E) Zebrafish lacking Mcm7 develop pericardial edema. 5 independent experiments. *P < 0.05, ***P < 0.0001. One-way ANOVA with Holm–Sidak's multiple comparison test. (C–E) Numbers of embryos analysed are given below the bars in E.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.