Fig. 5

Effect of a Notch signaling inhibitor, DAPT, on slurp-like1 expression. (A–D) Wild-type embryos at 6 hpf (A, B) were exposed to 100 μM DAPT (Notch signal inhibitor: B) or the same volume of DMSO (control: A) and incubated at 28.5 °C until 24 hpf, while wild-type embryos at 16 hpf (C, D) were incubated with 100 μM DAPT (D) or DMSO (C) and incubated until 24 hpf. The expression of slurp-like1 was examined by WISH using the antisense slurp-like1 RNA probe. The slurp-like1 expression in the hypochord (arrowhead) was strongly inhibited in the embryos treated with early DAPT treatment (B; 6 hpf), while its expression was partly inhibited in the embryos with late DAPT treatment (D; 16 hpf). The slurp-like1 expression in the floor plate (arrow) was not affected. Scale bars, 100 μm.