FIGURE

Fig. 3

ID

ZDB-FIG-181011-29

Publication

Zelinka et al., 2018 - Targeted disruption of the endogenous zebrafish rhodopsin locus as models of rapid rod photoreceptor degeneration.

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Fig. 3

Disruption of the N-linked glycosylation sequence in rho^fl10 and rho^fl7 leads to rod degeneration. A, C: Chromatograms overlaid with amino acid sequences comparing WT, rho^fl10, and rho^fl7 alleles, disrupting the conserved NXT consensus glycosylation sequence at N15. Red highlights deleted amino acids, while blue highlights insertions. B, D: Confocal images of serial retinal cryosections of 5 or 6 dpf WT or heterozygous rho^fl10/+ and rho^fl7/+ mutants labeled with antibodies to Rho (1D1, red), rods (4C12, red), and Arr3a (Zpr-1, red) overlaid with bright-field microscopy reveal loss of rod-specific labeling in the central retina. E: Higher magnification images of the dorsal retinal margin from WT, heterozygous rho^fl10/+, or rho^fl7/+ mutants showing sparse immunolabeling for Rho and rods.

Expression Data

Gene:	rho
Antibodies:	Ab-4C12 Ab1-rho
Fish:	rho^fl7/+ rho^fl10/+
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rho^fl7/+ rho^fl10/+
Observed In:	retinal rod cell retinal rod cell photoreceptor outer segment
Stage:	Day 6

Phenotype Detail

Acknowledgments

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