FIGURE

Fig. S5

ID

ZDB-FIG-180927-14

Publication

Hu et al., 2018 - Glypican 4 and Mmp14 interact in regulating the migration of anterior endodermal cells by limiting extracellular matrix deposition

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Fig. S5

Lam deposition is increased in gpc4 mutant embryos.

Transverse cryosections from Tg(sox17:EGFP) control and gpc4 mutant embryos immunostained for Lam (magenta) and nuclei (DAPI, blue). (A-D) Confocal z-stack images of embryos at tailbud (TB) (AB ) and 4-somite (4s) (C-D) stages. Lam assembly between the ectoderm and mesoderm (yellow arrowheads) and around the endodermal layer (white arrowheads). (E) Relative Lam intensity in nonendodermal (Non-end) tissue and around the endodermal layer (End) in control and gpc4 mutant embryos at TB and 4s. The number of embryo analyzed is shown in the graph. Bars represent the mean±s.e.m. *, P<0.05, student’s t-test.

Expression Data

Gene:	EGFP
Antibody:	Ab1-lam
Fish:	gpc4^fr6/fr6 ha01Tg gpc4^fr6/fr6; ha01Tg
Anatomical Terms:	anatomical line endodermal cell
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gpc4^fr6/fr6; ha01Tg
Observed In:	anatomical line
Stage:	Bud

Phenotype Detail

Acknowledgments

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