FIGURE

Fig. S6

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ZDB-FIG-180914-29

Publication

Rasmussen et al., 2018 - Fish Scales Dictate the Pattern of Adult Skin Innervation and Vascularization

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Fig. S6

Nerves and capillaries extend along radii of scales isolated from diverse body locations, Related to Figure 5

(A) Schematic indicating the different regions of the trunk from which scales were isolated in panel B. (B) DIC and fluorescent images of scales isolated from various regions of the trunk of adult female and male animals (25-27 mm SL). White lines and dashed boxes in top panels indicate scale margins and areas of magnification for lower panels, respectively. Arrows, superficial nerves associated with vasculature. Note several examples of radii that are occupied by nerves but not vasculature (arrowheads). Transgenes: (B) endothelium [Tg(fli1a:EGFP)] and axons [Tg(p2rx3a>mCherry)]. Scale bars, 500 μm (top panels) and 100 μm (lower panels).

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Cell, 46(3), Rasmussen, J.P., Vo, N.T., Sagasti, A., Fish Scales Dictate the Pattern of Adult Skin Innervation and Vascularization, 344-359.e4, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell