FIGURE

Fig. 4

ID

ZDB-FIG-180914-20

Publication

Rasmussen et al., 2018 - Fish Scales Dictate the Pattern of Adult Skin Innervation and Vascularization

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Fig. 4

Scales Vascularize Independently of Sensory Axons

(A–C) Representative lateral views of the trunk from wild-type and erbb3b^−/− adults. Arrows, superficial scale capillaries. Arrowheads, microvasculature associated with scale margin. Asterisk, interconnection of superficial capillary with scale margin vasculature. Dashed lines, scale margins. Double-headed arrow, example of dermal vasculature not associated with sensory nerves. Boxes in (C) indicate areas of magnification for (D)–(F).

(D and E) Magnification of scale capillaries. Note the two intertwined vessels in (D).

(F) Single z section through the tip of a scale capillary. Orthogonal views show that vessel lumens merge near posterior tip to form a U shape. Arrow, cell within the vessel lumen. Yellow lines in (D)–(F), planes of orthogonal sections.

(G–I) Representative scale capillaries immunostained for the indicated markers from adult wild-type, erbb3b^−/− and adgra2^−/− fish.

Transgenes: endothelium (Tg(fli1a:EGFP)); (A–C) axons (Tg(p2rx3a>mCherry)); (D) artery (Tg(-0.8flt1:RFP)) (Bussmann et al., 2010); lymphatic (TgBAC(prox1a:KALTA4,4xUAS-ADV.E1b:TagRFP)) (van Impel et al., 2014). Staining: (D) tight junctions (anti-Tjp1); (E) axons (acTubulin) and nuclei (DAPI); (F) F-actin (phalloidin), axons (acTubulin), and nuclei (DAPI); (G–I) F-actin (phalloidin), tight junctions (anti-Tjp1), axons (acTubulin), and nuclei (DAPI). Scale bars, 100 μm (A–C) and 10 μm (D–I). See also Figure S4.

Expression Data

Genes:	EGFP mCherry RFP
Antibodies:	Ab1-tjp1 Ab1-tuba
Fish:	adgra2^s984/s984 erbb3b^st48/st48 hu5333Tg; y1Tg la207Tg; y1Tg nim5Tg; y1Tg erbb3b^st48/st48; la207Tg; y1Tg
Anatomical Terms:	artery tight junction scale artery scale axon scale endothelial cell scale lymph vasculature scale vasculature vasculature tight junction
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	adgra2^s984/s984 erbb3b^st48/st48 erbb3b^st48/st48; la207Tg; y1Tg
Observed In:	scale axon scale vasculature
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 46(3), Rasmussen, J.P., Vo, N.T., Sagasti, A., Fish Scales Dictate the Pattern of Adult Skin Innervation and Vascularization, 344-359.e4, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell