FIGURE

Fig. S6

ID

ZDB-FIG-180905-29

Publication

Genthe et al., 2017 - R-spondin-1 is required for specification of hematopoietic stem cells through Wnt16 and Vegfa signaling pathways

Other Figures

All Figure Page

Back to All Figure Page

Fig. S6

Vegfa does not rescue dlc and dld expression in Rspo1 morphants. (A) Lateral view image of wild-type 17 hpf embryo with box indicating the location of in situ images. The cartoon shows a flat mount section of the tail of the embryo with formed somites and the pre-somitic mesoderm indicated. Wild-type (B,F), Rspo1 MO (C,G), Rspo1 MO + vegfaa-165 mRNA (D,H), or Rspo1 MO + vegfaa-121 mRNA (E,I) were examined by WISH for expression dlc (B-E) or dld (F-I). Numbers of embryos displaying the depicted phenotype are indicated. Magnification 160X. (J-K) dlc and dld mRNA (mean±SEM) levels relative to ef1 control quantified by qRT-PCR from uninjected (WT), Rspo1 MO Rspo1 MO + vegfaa-165 mRNA and Rspo1 MO + vegfaa-121 mRNA injected animals. * significantly different from WT (p ≤ 0.005) determined by one-way ANOVA.

Expression Data

Genes:	dlc dld
Fish:	WT WT + MO3-rspo1
Knockdown Reagent:	MO3-rspo1
Anatomical Terms:	segmental plate somite
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-rspo1
Knockdown Reagent:	MO3-rspo1
Observed In:	somite
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development